Chromatographic Separation of Naproxen Enantiomers using Hydroxypropyl‐β‐Cyclodextrin as Chiral Mobile Phase Additive

Abstract

Chromatographic separation of naproxen has been studied using hydroxypropyl‐β‐cyclodextrin as chiral mobile phase additive. The effects of mobile‐phase composition were researched in detail. The appropriate composition of mobile phase was 85:15 (v/v) aqueous with 0.5% TEA at pH 3.5/ ethanol containing 25 mM HP‐β‐CD, and the column temperature was set of 25°C. Graphs of 1/k versus [HP‐β‐CD] gave good linear relationships, indicating the stoichiometry ratio of naproxen with HP‐β‐CD of 1:1. Apparent thermodynamic parameters were also calculated from the plots of lnα versus 1/T, it was found that the enantioseparation was enthalpy driven and the inclusion process was exothermic.

Keywords:

• Chromatographic separation
• naproxen enantiomers
• hydroxypropyl‐β‐cyclodextrin

Acknowledgements

This work was supported by the Natural Science Foundation of China 20376085.