![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
Samples of hyaluronic acid (HA) produced by submerged fermentation using a synthetic culture medium were recovered, purified, fractioned, and characterized using solvent precipitation and size-exclusion chromatography (SEC). The samples showed a wide molar mass distribution in the range 103–107 Da, most of which had an average molar mass between 104 and 105 Da after purification by sequential precipitations. Fractions of HA with molar mass above 105 Da were purified by SEC in a semi-preparative scale with nearly no protein contamination. Characterization and fractionation of the HA was carried out by SEC in a analytical and semi-preparative scale using Shodex OHPak SB806M HQ and Superose 6 columns, respectively.
ACKNOWLEDGEMENTS
The authors wish to thank Dr. Maria Helena de Andrade Santana (Unicamp, Brazil) for providing samples of microbial HA produced at her laboratory. This study was supported by the Brazilian Ministry for Science and Technology – CNPq/PADCT 620118/2004-0, CNPq/FUNCAP 35.0134/2007-3 and CAPES/PRODOC 41/2005.
