ABSTRACT
The aim of the study was to determine the effect of selected variables on fermentation of decoction of Saraca asoca bark to develop a suitable method for preparing its polyherbal formulation. S. asoca bark has been fermented using Wickerhamomyces anomalus (nonsaccharomyces yeast) and the method was optimized using response surface methodology (RSM). The independent variables, substrate volume (ml), temperature (°C), and time (hours), were optimized using three-factor, three-level Box–Behnken design to obtain the good content of secoisolariciresinol, lyoniresinol, and 5-methoxy isolariciresinol. Secoisolariciresinol and 5-methoxy isolariciresinol were first isolated from S. asoca bark. The method yielded a quadratic polynomial equation to predict the effect of independent variables on selected responses (<0.0001). All three variables significantly (p-value < 0.0001–0.0068) affected the selected responses. The experimental values agreed closely with the predicted values and the analysis of variance signified a good model fit. The optimum conditions were 67.03 ml substrate volume, 30.95°C, and 64.86 h resulting in 23.24, 21.20, 25.06 μg of secoisolariciresinol, lyoniresinol, and 5-methoxy isolariciresinol content. This is the first report of biotechnological production of Asokarista using W. anomalus in 48 h rather than 35 days as mentioned in traditional method. Also, secoisolariciresinol has been found in fermented S. asoca bark for the first time. It has been ascertained that W. anomalus causes deglycosylation in traditional ayurvedic formulations during fermentation. Present study proved the possibility of using pure strain, Wi. anomalus, for formulating traditional formulation.
Funding
This work is supported by the grant from Department of Science and Technology, Ministry of Home Affairs, Government of India (SR/WOS-A/LS-119/2011).
Declaration of Interest
The authors declare that none of the competing interests exist for the article. The authors solely are responsible for the content and writing of the article.