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Separation Science and Technology Volume 52, 2017 - Issue 5
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Extraction

Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system

Hadi SofijanInstitute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia;Department of Chemistry, Faculty of Science and Technology, Airlangga University, Surabaya East Jawa, Indonesia
,
Tan Joo ShunBioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia
,
Sahar AbbasiliasiLaboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia, Selangor, Malaysia
,
Shuhaimi MustafaLaboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia, Selangor, Malaysia
,
Nyoman Tri PuspaningsihDepartment of Chemistry, Faculty of Science and Technology, Airlangga University, Surabaya East Jawa, Indonesia
,
Saeid KadkhodaeiInstitute of Tropical Agriculture, Universiti Putra Malaysia, Selangor, Malaysia
&
Arbakariya B. AriffInstitute of Bioscience, Universiti Putra Malaysia, Selangor, Malaysia;Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Sciences, Universiti Putra Malaysia, Selangor, MalaysiaCorrespondence[email protected]
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Pages 834-842 | Received 19 Jan 2016, Accepted 30 Nov 2016, Published online: 11 Jan 2017
 
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ABSTRACT

A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant Bacillus megaterium MS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL), volume ratio (VR), crude loading and pH on the recovery performance was evaluated. Under the optimal extraction conditions, β-xylosidase was successfully purified up to 23-fold with a recovery yield of 99% in the bottom salt-rich phase at PEG 4,000/potassium phosphate ATPS comprising TLL of 41.8, VR of 2.3, crude loading (CL) of 30% (w/w) at pH 6.

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