A simple method for purification of bromelain in a thermosensitive triblock copolymer-based protection system and recycling of phase components

ABSTRACT

In the work, we chose stem bromelain as a model to investigate the storage and purification of bromelain from pineapple peel. Extraction of bromelain from pineapple peel using a two-stage aqueous two-phase extraction system composed of a thermoseparating copolymer EOPOEO and K₂HPO₄. Bromelain predominantly partitioned to the EOPOEO-rich phase and then re-extract to the top dilute phase. The recovery of enzyme activity (68.6%) and purification factor (6.53) were determined under optimum conditions. The EOPOEO-rich phase and salt were recycled, and the recovery of enzyme activity could reach up to 60%. This method has been proved to obtain highly purified and stable bromelain.

KEYWORDS:

• Aqueous two-phase systems
• thermoseparating polymers
• recycle
• extraction

Funding

This work was supported by the National Natural Science Foundation of China: [Grant numbers 31470434, 21406090, 21576124 and 21676124] and Project of the Jiangsu Higher Education Institutions of China: [Grant number 16KJB550010].

Additional information

Funding

This work was supported by the National Natural Science Foundation of China: [Grant numbers 31470434, 21406090, 21576124 and 21676124] and Project of the Jiangsu Higher Education Institutions of China: [Grant number 16KJB550010].