Immunomagnetic separation of B type acute lymphoblastic leukemia cells from bone marrow with flow cytometry validation and microfluidic chip measurements

ABSTRACT

In order to detect the blast cells from bone marrow of patients, one strategy is to first isolate the cells using immunomagnetic beads. The aim of this study was to report the experimental results of the immunomagnetic separation efficiency of the blast cells from bone marrow of pediatric leukemia patients. To test the efficiency of immunomagnetic separation, flow cytometry measurements at critical steps were performed. We here reported 94.5% capture efficiency for CD10 nano beads. Patients samples were also analyzed with a microfluidic chip to test the feasibility for further developments.

KEYWORDS:

• B type acute lymphoblastic leukemia
• immunomagnetic separation
• flow cytometry
• bone marrow
• hematogone
• microfluidic chip

Acknowledgments

Authors acknowledge The Scientific and Technological Research Council of Turkey (TÜBİTAK Project No: 115E020) for financial support.

Author contributions

K.I., E.U., S.O. designed the experiments, A.M., S.O., E.U. and K.I conducted the experiments, S.C., A.E. G.D. conducted FC measurements and analyzed the data, all authors interpreted the data and wrote the manuscript.

Additional information

Funding

This work was supported by the Türkiye Bilimsel ve Teknolojik Araştirma Kurumu [project number: 115E020].