Modification of the Capillary Zone Electrophoretic Retention Behavior of Deoxyribonucleotides by Different Mobile Phases

Abstract

The effect of the addition of isopropanol as a organic modifier in capillary zone electrophoresis is discussed in terms of resolution using the four deoxyribonucleotides of dAmp, dGmp, dTmp, and dCmp. The system consisted of 60-μm i.d. fused silica tubing and 68.5 cm column length, a high voltage power supply, and a UV detector set at 254 nm. The effect of the eight different mobile phases on the column efficiency and resolution were compared to find the optimum mobile phase. The experimental results showed that the mobile phase of phosphate/Tris with 0.05 M SDS was most suitable for the separation of the components, and that the column efficiency and resolution were affected by the separation voltage.