LINC00943 knockdown attenuates MPP⁺-induced neuronal damage via miR-15b-5p/RAB3IP axis in SK-N-SH cells

ABSTRACT

Objectives

Parkinson’s disease (PD) is a neurodegenerative problem correlated with neuronal damage. Long noncoding RNAs (lncRNAs) are implicated in neuronal damage in PD development. This research aims to analyze the function and mechanism of LINC00943 in 1-methyl-4-phenylpyridinium (MPP⁺)-caused neuronal injury.

Methods

MPP⁺-challenged SK-N-SH cells served as a PD-like model of neuronal damage. LINC00943, microRNA-15b-5p (miR-15b-5p) and RAB3A interacting protein (RAB3IP) abundances were examined via quantitative reverse transcription polymerase chain reaction or western blot. MPP⁺-caused neuronal damage was assessed via cell viability, apoptosis, inflammatory injury and oxidative injury. The association between miR-15b-5p and LINC00943 or RAB3IP was determined via dual-luciferase reporter analysis and RNA immunoprecipitation.

Results

LINC00943 abundance was up-regulated in MPP⁺-challenged SK-N-SH cells. LINC00943 silence alleviated MPP⁺-caused decrease of cell viability and elevation of apoptosis, inflammatory injury and oxidative injury. miR-15b-5p was inhibited via LINC00943, and miR-15b-5p inhibition reversed knockdown of LINC00943-mediated suppression of MPP⁺-induced neuronal damage. RAB3IP was targeted via miR-15b-5p, and LINC00943 could regulate RAB3IP via miR-15b-5p. miR-15b-5p addition mitigated MPP⁺-induced neuronal damage through decreasing RAB3IP.

Conclusion

LINC00943 inhibition alleviated MPP⁺-induced neuronal injury via miR-15b-5p/RAB3IP axis, indicating a potential target for treatment of PD.

KEYWORDS:

• Parkinson’s disease
• MPP⁺
• neuronal damage
• LINC00943
• miR-15b-5p
• RAB3IP

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Notes on contributors

Chunming Meng

Chunming Meng, Department of Geriatrics, The First People's Hospital of Lianyungang, Research direction is Geriatric Nursing.

Jing Gao

Jing Gao, Department of Geriatrics, The First People's Hospital of Lianyungang, Research direction is Geriatric Nursing.

Qingyuan Ma

Qingyuan Ma, Department of Geriatrics, The First People's Hospital of Lianyungang, Research direction is Geriatric Nursing.

Qian Sun

Qian Sun, Department of Geriatrics, The First People's Hospital of Lianyungang, Research direction is Geriatric Nursing.

Tiantian Qiao

Tiantian Qiao, Department of Geriatrics, The First People's Hospital of Lianyungang, Research direction is Geriatric Nursing.