Isocyanate induces cytotoxicity via activation of phosphorylated alpha synuclein protein, nitrosative stress, and apoptotic pathway in Parkinson’s Disease model-SHSY-5Y cells

ABSTRACT

Background

Neurotoxic disorders account for a significant portion of the diseases that influence the worldwide disease burden. Parkinson’s disease is one such disease that is linked with environmental toxin exposure. Isocyanates are a highly reactive industrial intermediate used widely in manufacturing plastic products, paints, etc. This study aims to delineate the neurotoxic potential of isocyanate in Parkinson’s cell model-SHSY-5Y cells.

Methodology

SHSY-5Y cells were treated with isocyanate analogue (N succinimidyl N methyl carbamate) in time and dose dependant manner. Different parameters were assessed like protein expression, nitrosative stress level, antioxidant enzymes level and apoptosis.

Results

Our findings demonstrate that dose- and time-dependent isocyanate exposure increases reactive nitrogen species and decreases the glutathione, SOD, and catalase levels. Further, increased phosphorylated alpha-synuclein protein and activation of caspase 3 exert cytotoxicity in SHSY-5Y cells.

Conclusion

Our research reveals that widely used isocyanate induces cytotoxicity, apoptosis, nitrosative stress, and protein dysfunction, which might constitute a potential mechanism of neurodegeneration in Parkinsonism.

KEYWORDS:

• Environmental exposure
• N-succinimidyl N-methyl carbamate
• caspase 3
• alpha synuclein protein
• Parkinson‘s disease
• nitorsative stress

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The author(s) reported that there is no funding associated with the work featured in this article.