Evaluation of peripheral nerve injury according to the severity of damage using ¹⁸F-FDG PET/MRI in a rat Model of sciatic nerve injury

ABSTRACT

Objectives

We ascertained that the PET scan may be a valuable imaging modality for the noninvasive, objective diagnosis of neuropathic pain caused by peripheral nerve injury through the previous study. This study aimed to assess peripheral nerve damage according to severity using¹⁸F-FDG PET/MRI of the rat sciatic nerve.

Methods

Eighteen rats were divided into three groups: 30-second (G1), 2-minute (G2), and 5-minute (G3) crushing injuries. The severity of nerve damage was measured in the third week after the crushing injury using three methods: the paw withdrawal threshold test (RevWT), standardized uptake values on PET (SUVR), and intensity analysis on immunohistochemistry (IntR).

Results

There were significant differences between G1 and G3 in both SUVR and IntR (p = 0.012 and 0.029, respectively), and no significant differences in RevWT among the three groups (p = 0.438). There was a significant difference in SUVR (p = 0.012), but no significant difference in IntR between G1 and G2 (p = 0.202). There was no significant difference between G2 and G3 in SUVR and IntR (p = 0.810 and 0.544, respectively).

Discussion

Although PET did not show results consistent with those of immunohistochemistry in all respects, this study demonstrated that PET uptake tended to increase with severe nerve damage. If this research is supplemented by further experiments, PET/MRI can be used as an effective diagnostic modality.

KEYWORDS:

• Peripheral nerve injury
• crushing injury
• ¹⁸F-fluorodeoxyglucose (¹⁸F-FDG)
• positron emission tomography (PET)
• magnetic resonance imaging (MRI)

Disclosure statement

No potential conflict of interest was reported by the author(s).

Ethical approval

All procedures involving animals were performed in accordance with the ethical standards of the Institutional Animal Use and Care Committee of the Department of Laboratory Animal Resources, Yonsei Biomedical Research Institute, Yonsei University College of Medicine, Korea.

Additional information

Funding

This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) (No. 2019R1G1A1085187).