Lycium Europaeum Fruit Extract: Antiproliferative Activity on A549 Human Lung Carcinoma Cells and PC12 Rat Adrenal Medulla Cancer Cells and Assessment of Its Cytotoxicity on Cerebellum Granule Cells

Abstract

Cancer is a major worldwide health problem and one of the leading causes of death either in developed or developing countries. Plant extracts and derivatives have always been used for various disease treatments and many anticancer agents issued from plants and vegetables are clinically recognized and used all over the world. Lycium europaeum (Solanaceae) also called “wolfberry” was known since ancient times in the Mediterranean area as a medicinal plant and used in several traditional remedies. The Lycium species capacity of reducing the incidence of cancer and also of halting or reserving the growth of cancer was reported by traditional healers. In this study, the antiproliferative capacity, protective properties, and antioxidant activity of the hydro-alcoholic fruit extract of Lycium europaeum were investigated. Results showed that Lycium extract exhibits the ability to reduce cancer cell viability, inhibits proliferation, and induces apoptosis in A549 human lung cancer cells and PC12 rat adrenal medulla cancer cells, in a concentration- and time-dependent manner. Cytotoxic effect on normal rat cerebellum granule cells was assessed to be nonsignificant. Results also showed that Lycium fruit extract protected lipids, proteins, and DNA against oxidative stress damages induced by H₂O₂ via scavenging reactive oxygen species.

ACKNOWLEDGMENTS

This work was supported by the Tunisian Ministry of High Education and Scientific Research, INSERM, and Rouen University. We thank all the team of the U INSERM 982 Différenciation & Communication Neuronale & Neuroendocrine Laboratory and the team of the PISSARO proteomic facility (IRIB) for all their help.