Fe₃O₄ Nanopowders: Genomic and Apoptotic Evaluations on A549 Lung Adenocarcinoma Cell Line

Abstract

The magnetite nanoparticles are progressively used in a wide range of biological applications. In the present study, we purposed to show apoptosis-inducing ability of Fe₃O₄ nanopowders on A549 cells. In addition, the toxic effects of Fe₃O₄ nanopowders were researched on L929 cells. The cytotoxicity of Fe₃O₄ nanopowders were evaluated on A549 and L929 cells by MTT assay and inhibited cell proliferation by time and dose-dependent manner on A549 cells but was not toxic on L929 cells. According to these findings, IC₃₀ value of Fe₃O₄ nanopowders was determined as 5 µM. The early and late apoptotic cells were detected by Annexin V-FITC/PI assay using IC₃₀ concentration of Fe₃O₄ nanopowders. Furthermore, The IC₃₀ value of Fe₃O₄ nanopowders was not effective in the activation of caspase-3 but was effective on loss of mitochondrial membrane potential. The apoptotic index of A549 cells was investigated and found out to increase by IC₃₀ value of Fe₃O₄ nanopowders using TUNEL, BrdU, Bcl-2 immunocytochemical assays. The upregulated and downregulated genes were profiled and the presence of some apoptotic genes was determined with administration of IC₃₀ value of Fe₃O₄ nanopowders by microarray assay. This work suggests that Fe₃O₄ nanopowders could be a good candidate for therapy of lung adenocarcinoma cells.

Acknowledgments

This article does not include any studies with human participants or animals. We thanks to Medical Plants, Drugs and Scientific Research Center for cell culture studies at Anadolu University.

Disclosure Statement

The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Additional information

Funding

This work was supported by Anadolu University, Scientific Research Projects (no. 1305F088).