http://orcid.org/0000-0002-2159-8262
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Abstract
The change of fatty acid composition has been regarded as an indicator of altered lipid metabolism during human tumourigenesis, but the details are still unclear. We have previously demonstrated a monounsaturated fatty acid (MUFA) named oleic acid (OA) was involved in renal cell carcinoma (RCC) cell growth, as an extracellular signaling molecule to regulate 786-O cell proliferation via the integrin-linked kinase (ILK) pathway. In this study, we further observe the effects of OA on cell invasion of RCC and the potential mechanism by which OA worked was determined. The transwell invasion assay showed OA increased cell invasion of RCC in a dose-dependent manner. Western blotting results indicated ILK, COX-2, and MMP-9 proteins were involved for their high expressions and these effects were reversed when down-regulating the expression of ILK by special siRNA. The MMPs inhibitor GM6001 could weaken the abilities of OA on RCC cells invasion. These results suggested MUFA indeed affected cell invasion of RCC, which was depended by the regulation of ILK pathway.
Acknowledgments
The authors would like to thank Dr. Jing Li for her excellent technical assistance.
Disclosure Statement
The authors have no conflict of interest to declare.
Additional information
Funding
Notes on contributors
Zhen-Hua Liu
Dr. Zhen-hua Liu, Dr. Cai-peng Qin, and Dr. Zheng-zuo Sheng performed the research program and gathered the whole data; Dr. Zhen-hua Liu drafted the article and analyzed the data; Prof. Gui-zhong Li and Prof. Li-bo Man revised the article critically; Prof. Tao Xu designed the program and was responsible for theoretical guidance. All authors approved the final version of the manuscript accepted for publication.
Cai-Peng Qing
Dr. Zhen-hua Liu, Dr. Cai-peng Qin, and Dr. Zheng-zuo Sheng performed the research program and gathered the whole data; Dr. Zhen-hua Liu drafted the article and analyzed the data; Prof. Gui-zhong Li and Prof. Li-bo Man revised the article critically; Prof. Tao Xu designed the program and was responsible for theoretical guidance. All authors approved the final version of the manuscript accepted for publication.
Zheng-Zuo Sheng
Dr. Zhen-hua Liu, Dr. Cai-peng Qin, and Dr. Zheng-zuo Sheng performed the research program and gathered the whole data; Dr. Zhen-hua Liu drafted the article and analyzed the data; Prof. Gui-zhong Li and Prof. Li-bo Man revised the article critically; Prof. Tao Xu designed the program and was responsible for theoretical guidance. All authors approved the final version of the manuscript accepted for publication.
Gui-Zhong Li
Dr. Zhen-hua Liu, Dr. Cai-peng Qin, and Dr. Zheng-zuo Sheng performed the research program and gathered the whole data; Dr. Zhen-hua Liu drafted the article and analyzed the data; Prof. Gui-zhong Li and Prof. Li-bo Man revised the article critically; Prof. Tao Xu designed the program and was responsible for theoretical guidance. All authors approved the final version of the manuscript accepted for publication.
Li-Bo Man
Dr. Zhen-hua Liu, Dr. Cai-peng Qin, and Dr. Zheng-zuo Sheng performed the research program and gathered the whole data; Dr. Zhen-hua Liu drafted the article and analyzed the data; Prof. Gui-zhong Li and Prof. Li-bo Man revised the article critically; Prof. Tao Xu designed the program and was responsible for theoretical guidance. All authors approved the final version of the manuscript accepted for publication.
Tao Xu
Dr. Zhen-hua Liu, Dr. Cai-peng Qin, and Dr. Zheng-zuo Sheng performed the research program and gathered the whole data; Dr. Zhen-hua Liu drafted the article and analyzed the data; Prof. Gui-zhong Li and Prof. Li-bo Man revised the article critically; Prof. Tao Xu designed the program and was responsible for theoretical guidance. All authors approved the final version of the manuscript accepted for publication.