Fish Lipid against Prostate Cancer (PC-3) through Apoptosis and Cell Cycle Arrest

Abstract

Anti-proliferative and apoptotic activities of different concentrations (10-50 μg/ml) of total lipid of the freshwater fish, Labeo rohita against human prostate cancer cells (PC3) were assessed using cells viability analysis by MTT assay, intracellular ROS generation and nuclear condensation. The cell cycle analysis for DNA content was performed by flow cytometry. The fish lipid was found to be effective which changed the characteristic morphology of PC3cells and also decreased their cells number. The fish lipid significantly induced the cell cycle arrest and level of ROS which caused apoptosis in PC3cells. The anti-proliferative and apoptotic roles of the fish lipid against the cells of prostate cancer may be helpful for the prevention and development of anticancer drug.

Acknowledgments

The authors thank the Head, Department of Zoology of University of Lucknow for the facility and administrative support. Author, Pragya Gupta is also thankful to University Grant Commission for UGC-BSR fellowship. The study was funded by UPCST, Lucknow. The authors also wish to acknowledge Mr. AL Vishwakarma, SAIF-CDRI, Lucknow for technical assistance for FACS analysis.

Disclosure Statement

There were no conflicts of interest between authors.

Author’s Contribution

PG did the experiment, data collection and analysis; PG and MS did the conceptualization, writing and critical revision of the manuscript.