NADPH‐cytochrome P‐450 reductase, cytochrome P‐450 2C11 and P‐450 1A1, and the aryl hydrocarbon receptor in livers of rats fed methyl‐folate‐deficient diets

Jianzhong Zhang Clinical Nutrition Research Unit, Department of Medicine, University of California, Los Angeles, CA, 90095

Susanne M. Henning Clinical Nutrition Research Unit, Department of Medicine, University of California, Los Angeles, CA, 90095

David Heber Clinical Nutrition Research Unit, Department of Medicine, University of California, Los Angeles, CA, 90095

Jiye Choi Clinical Nutrition Research Unit, Department of Medicine, University of California, Los Angeles, CA, 90095

Yuhua Wang Clinical Nutrition Research Unit, Department of Medicine, University of California, Los Angeles, CA, 90095

Marian E. Swendseid Clinical Nutrition Research Unit, Department of Medicine, University of California, Los Angeles, CA, 90095

Vay Liang W. Go Clinical Nutrition Research Unit, UCLA School of Medicine, Warren Hall, Rm. 13–146, 900 Veteran Ave., Los Angeles, CA, 90095–1786 Phone: (310) 794–7848 Fax: (310) 794–7848 E-mail: [email protected]

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Abstract

We investigated three hepatic cytochrome P‐450 isozymes and the aryl hydrocarbon (Ah) receptor in rats fed one of the following three diets for 15 months: a diet containing the AIN vitamin mixture (control), the control diet devoid of choline andfolate (CFD), or the CFD diet devoid of niacin (CFND). Hepatic tumors developed in all CFD‐and CFND‐fed rats. Western blot analyses of nontumor hepatic tissue showed that NADPH‐cytochrome P‐450 reductase (P‐450 reductase) increased significantly in the CFD and CFND groups compared with the control group. Hepatic cytochrome P‐450 2C11 (CYP2C11) was not detectable in the CFD and CFND groups compared with the control group. Ah receptor and cytochrome P‐450 1A1 (CYP1A1) were detected in higher amounts in livers of both deficient groups. CYP1A1 is an enzyme associated with bioactivation of exogenous genotoxins. To our knowledge, this is the first time it has been shown that CYP1A1 and the Ah receptor are induced by dietary deficiencies.

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NADPH‐cytochrome P‐450 reductase, cytochrome P‐450 2C11 and P‐450 1A1, and the aryl hydrocarbon receptor in livers of rats fed methyl‐folate‐deficient diets

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NADPH‐cytochrome P‐450 reductase, cytochrome P‐450 2C11 and P‐450 1A1, and the aryl hydrocarbon receptor in livers of rats fed methyl‐folate‐deficient diets

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