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Summary
A simple, mechanical method is described for the isolation of preantral follicles from bovine foetuses of 220–280 days of gestation. On average, 2918 + 621 (s.d.) preantral follicles were isolated per ovary. The isolated preantral follicles were characterized on the basis of the morphological appearance of the surrounding granulosa cells, the number of granulosa cell layers, and their diameter. The results show that primordial, primary, and secondary follicles differ morphologically and that they can be classified by their diameter.
Notes
Department of Functional Morphology, Faculty of Veterinary Medicine, University of Utrecht. Yalelaan 1, 3508 TD, Utrecht. The Netherlands.
Department of Animal Edoncrinolgy and Reproduction (IRSIA Research Unit), B41 Faculty of Veterinary Medicine, State University of Liège, B‐4000 Liège, Belgium.
Department of Herd Health and Reproduction. Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 8, 3508 TD. Utrecht, the Netherlands.