Dopaminergic neurons can influence heat-box place learning in Drosophila

Abstract

Dopamine provides crucial neuromodulatory functions in several insect and rodent learning and memory paradigms. However, an early study suggested that dopamine may be dispensable for aversive place memory in Drosophila. Here we tested the involvement of particular dopaminergic neurons in place learning and memory. We used the thermogenetic tool Gr28bD to activate protocerebral anterior medial (PAM) cluster and non-PAM dopaminergic neurons in an operant way in heat-box place learning. We show that activation of PAM neurons influences performance during place learning, but not during memory testing. These findings provide a gateway to explore how dopamine influences place learning.

Keywords:

• Dopamine
• place learning
• PAM
• heat-box
• valence

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This paper is dedicated to our late mentor, and friend, Troy Zars. We are grateful to Bertram Gerber and Scott Waddell for their valuable inputs into crafting this paper. This work was supported by NSF Grant 1535790 to T.Z.