Modification of titanium surface with collagen and doxycycline as a new approach in dental implants

Abstract

In this work, we investigate for the first time several issues involved in bio-adhesion process for a new type of chemically modified titanium surfaces (in their initial form and after collagen deposition), in order to assess their potential in dental implant surface modification. For this purpose, we studied the following: collagen adhesion, cytotoxicity, osteoblast cytomorphology, cell adhesion and proliferation, doxycycline embedding and modifications in the collagen film deposed on the metal surfaces, drug release from the collagen films. The improvement of adhesion between collagen film and titanium substrate, when hydroxyl and amino functional groups are assisting the surfaces was presented, all materials showing no cytotoxic effects as revealed by lactate dehydrogenase-based assay. The drug release from titanium–coll–doxy systems offers a dual mechanism of the delivery profile (burst release followed by moderate discharge of the antibiotic), with perspectives in soft tissue recovery postoperative stage.

Keywords:

• titanium
• collagen
• doxycycline
• drug delivery
• osseointegration

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the ‘Parteneriate in domenii prioritare – PNII’, supported by MEN-UEFISCDI [project no. 155/2014].