Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose

Abstract

The effect of different vitamins and inorganic micronutrients on callus growth and on the induction and proliferation of somatic embryos from young mature, fully expanded leaves of rose (Rosa hybrids L.) was investigated. Expiants were cultured onto a solid Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose, 53.5 μM p‐chlorophenoxyacetic acid, 4.6 μM Kinetin, and 0.5 mg L⁻¹ thiamin.HCI in combination with either of eleven different vitamins. Alternatively, expiants were cultured onto a solid medium containing MS macro‐ and micronutrients except for the salts of manganese (Mn), zinc (Zn), iodine (I), copper (Cu), and cobalt (Co), which were added at either the standard MS concentration or at a tenfold increased (Cu, Co) or decreased (Mn, Zn, I) concentration. Embryo maturation and germination took place on a MS medium supplemented with 5.2 μM 6‐benzyladenine and 5.7 μM 3‐indole‐acetic acid. The results indicated that somatic embryogenesis from rose leaves was favored by the addition of nicotinic acid and cystein to the culture medium and the increase of Cu concentration, without reducing embryo maturation and germination.

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