Interaction Between Phosphorus Nutrition and Drought on Grain Yield, and Assimilation of Phosphorus and Nitrogen in Two Soybean Cultivars Differing in Protein Concentration in Grains

ABSTRACT

Drought affects many physiological and biochemical processes and thus reduces plant growth. Phosphorus (P) fertilization improves tolerance to drought stress in many plants. A greenhouse experiment examined the interactive effects of P nutrition and drought stress on P accumulation and translocation, yield, and protein concentration in grains of two cultivars of soybean [Glycine max (L.) Merr.]. Plants of cultivars ‘Heisheng 101’ (high protein in grains) and ‘Dongnong 464’ (low protein) were grown in a P-deficient soil supplied with 0–30 mg P kg⁻¹ soil. Drought stress was imposed at the initial flowering (R1) or the podding (R4) stage. Drought stress limited P accumulation and reduced P translocation to the seed. The addition of P enhanced the concentration and accumulation of nitrogen (N) and P in shoots and seeds of both cultivars. Drought stress decreased shoot biomass, grain yield, and P accumulation; the decrease was greater in ‘Dongnong 46’ than ‘Heisheng 101,’ and even more so if drought stress was imposed at R4 than at R1. In contrast, drought stress increased the concentration of N in shoot and protein in grains. The addition of P alleviated the effect of drought stress on plant growth, P accumulation, and grain yield in both cultivars but to a greater extent in ‘Dongnong 46’. The results suggest that application of P fertilizers could mitigate drought stress at the reproductive stage, resulting in less yield penalty and improvement of grain quality of soybean grown in P-deficient soils.

Keywords:

• phosphorus
• drought stress
• yield
• protein
• cultivar variation

ACKNOWLEDGMENT

This work is supported by the Innovation Project (KZCX3-SW-NA-04) funded by the Chinese Academy of Sciences and Director Project (SB06B35) funded by Key Laboratory of Soybean Biology of Ministry of Education, China.

Notes

∗ P ≤ 0.05;

∗∗P ≤ 0.01;

∗∗∗P ≤ 0.001; n.s., not significant.

∗P response (%) is the relative seed yield using the yield of plants receiving no P as 100%.

∗Apparent P fertilizer recovery = (Pt_P−Pt₀)× 100/amount of P fertilized, where Pt_p and Pt₀ are total P uptake with and without P supply, respectively.

∗∗P-use efficiency (PUE) = grain yield/total P in biomass at maturity.