Role of Phosphatases, Iron Reducing, and Solubilizing Activity on the Nutrient Acquisition in Mixed Cropped Peanut and Barley

ABSTRACT

The effect of interspecific complementary and competitive root interactions and rhizosphere effects on primarily phosphorus (P) and iron (Fe) but also nitrogen (N), potassium (K), calcium (Ca), zinc (Zn), and manganese (Mn) nutrition between mixed cropped peanut (Arachis hypogaea L.) and barley (Hordeum vulgare L.). In order to provide more physiological evidence on the mechanisms of interspecific facilitation, phosphatase activities in plant and rhizosphere, root ferric reducing capacity (FR), Fe-solubilizing activity (Fe-SA), and rhizosphere pH were determined. The results of the experiment revealed that biomass yield of peanut and barley was decreased by associated plant species as compared to their monoculture. Rhizosphere chemistry was strongly and differentially modified by the roots of peanut and barley and their mixed culture. In the mixed cropping of peanut/barley, intracellular alkaline and acid phosphatases (AlPase and APase), root secreted acid phosphatases (S-APase), acid phosphatases activity in rhizosphere (RS-APase), and bulk soil (BS-APase) were higher than that of monocultured barley. Regardless of plant species and cropping system, the rhizosphere pH was acidified and concomitantly to this available P and Fe concentrations in the rhizosphere were also increased. The secretion Fe-solubilizing activity (Fe-SA) and ferric reducing (FR) capacity of the roots were generally higher in mixed culture relative to that in monoculture treatments which may improve Fe and Zn nutrition of peanut. Furthermore, mixed cropping improved N and K nutrition of peanut plants, while Ca nutrition was negatively affected by mixed cropping.

Keywords:

• barley
• ferric reducing capacity
• mixed cropping
• peanut
• phosphatases
• Fe(III)-solubilizing activity
• rhizosphere

Notes

∗: P < 0.05,

∗∗: P < 0.01, ns: non-significant.

∗: P < 0.05

∗∗: P < 0.01, ns: non-significant.

∗: P < 0.05,

∗∗: P < 0.01, ns: non-significant.

∗: P < 0.05,

∗∗: P < 0.01, ns: non-significant.