Nitrogen, Phosphorus, and Sulfur Nutrition in Broccoli Plants Grown Under Salinity

ABSTRACT

Broccoli (Brassica oleracea L. var. ‘Italica’) is a recognized health-promoting vegetable and shows a moderate sensitivity to salinity. As very little is known about the effect of salt stress on broccoli plants, the objective was to evaluate nitrogen (N), phosphorous (P), and sulfur (S) nutrition in plants grown under saline conditions. For this objective, the contents of nitrate, phosphate, and sulfate, and total nitrogen, phosphorus, and sulfur, as well as related metabolic enzymes, were determined for plants grown with 0, 20, 40, 60, 80, or 100 mM sodium chloride (NaCl) for two weeks. Nitrate, phosphate, and sulfate concentration in leaves and roots showed a maximum at 40–60 mM NaCl. Up to these salt levels, broccoli plants showed a normal development, but over these salt levels, broccoli plants showed a decrease of nitrate reductase and an increase of the acid phosphatase. From 60 to 100 mM NaCl, the nutritional disorders indicated that the threshold of resistance was exceeded.

Keywords:

• Broccoli
• salt stress
• nitrogen
• phosphorous
• sulfur
• nitrate reductase
• acid phosphatase
• O-acetylserine (thiol) lyase

ACKNOWLEDGMENTS

The authors wish to thank Dr. D Walker for correction of the written English in the manuscript. C. López-Berenguer was funded by a grant from Comunidad Autónoma de la Región de Murcia (Spain). This work was funded by CICYT AGL2006-06499/AGR).

Notes

∗∗∗: Significance at 0.01% level