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Abstract
In-vitro antioxidant properties of various extracts prepared by reflux, maceration and soxhelet methods from the leaves of Artemisia aucheri from Kashan, Central Iran, have been evaluated in this study. The effects of different solvents and extraction methods used were investigated qualitatively. Samples were collected from the field plots in a completely randomized (CR) design with three replications. Samples’ antioxidant potentials were evaluated using inhibition of free radical, DPPH (2,2-Di-Phenyl-1-Pycril Hydrazil), and also β-Carotene/Linoleic acid assay. Amongst various extracts used (water, Methanol, Chloroform, and n-Hexane), water extracts reduced the free radical more than any others, which was mostly higher than that of synthetic antioxidant BHT (Buthylated hydroxy toluene), with = 14.9 μg ml−1. Water extracts also showed major effects in β-Carotene/Linoleic acid assay with average inhibition of 61.73%. The results of this study show that maceration method offers the highest quality extract with regard to antioxidant activities.