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Research Articles

Effects of different NO3: NH4+ ratios on the ultrastructure and ion flux rate of lettuce roots

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Pages 2528-2545 | Received 08 Jul 2020, Accepted 08 Mar 2021, Published online: 21 Apr 2021
 

Abstract

Nitrogen is a vital nutrient element for plants. The two main forms of nitrogen (N) are nitrate N (NO3) and ammonium N (NH4+). The effects of the different NO3:NH4+ ratios (0:100, 25:75, 50:50, 75:25, and 100:0) in the nutrient solution on the growth, root morphology, ultrastructure and ion flux rate of lettuce roots were studied. The results showed that the biomass and leaf area were greatest when the ratio of NO3ˉ:NH4+ was 50:50 or 75:25. The shoot NO3 content was highest when the ratio of NO3ˉ:NH4+ was 50:50 or 75:25. The shoot NH4+-N content was highest when the ratio of NO3ˉ:NH4+ was 50:50. The NO3 flux was greatest when the ratio of NO3ˉ:NH4+ was 50:50 or 75:25. Superoxide dismutase (SOD) activity was lowest when the ratio of NO3ˉ:NH4+ was 0:100, and peroxidase (POD) activity was highest when the ratio of NO3ˉ:NH4+ was 100:0. The malonic dialdehyde (MDA) content was lowest when the ratio of NO3ˉ:NH4+ was 50:50, and the MDA content was higher when the ratio of NO3ˉ:NH4+ was 0:100 or 25:75. It was smooth and complete when the ratio of NO3ˉ:NH4+ was 50:50 or 75:25.

Disclosure statement

No potential conflict of interest was reported by the authors.

Authors contribution

Kaikai Du carried out the experiments and collected and analyzed the results. Chaojie Liu and Shuangxi Fan designed the experiments, Yingyan Han, Jinghong Hao, Xiaoxiao Qin and Chaojie Liu aided in the analysis of the results and editing of the manuscript. All authors contributed significantly, and all authors were in agreement with the content of the manuscript.

Additional information

Funding

The work was supported by the National Key Research and Development Program of China (2016YFD0201010), the Beijing Innovation Consortium of Agriculture Research Systems (BAIC07-2020) and the Science and Technology Program of the Beijing Municipal Education Commission (KM201910020012).

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