Abstract
The mechanism of radial transport of phosphate in corn roots was studied and we applied a 32P/33P double‐labelled method to estimate the specific activities of phosphate compounds in roots. In a previous paper, we showed that the radial transport of phosphate in roots was closely related to the turnover of glycolysis and that G6P played a particularly important role in the xylem loading process of phosphate from the stele.
In this report, the effects of glucosamine on phosphate metabolism in roots are discussed. The 32P/33P double‐labelled experiments and 31P‐NMR measurements showed that glucosamine inhibited the transport of phosphate to the shoots, followed by a great accumulation of vacuolar inorganic phosphate in stele cells.
The histochemical demonstration of glucose‐6‐phosphatase (3,1,3,9) revealed that the enzyme was localized in the stele and endodermis cells, especially along metaxylem and protoxylem.