Abstract
Using standard microculture techniques for propagating potato (Solatium tuberosum), the effects of several P and Mn treatments were investigated. Foliar yields were affected by both the P and Mn content of the media, but the major change observed was in nutrient uptake. Tissue levels of P and Mn changed with their respective treatments, and over a range of levels, Mn uptake increased with an increase in phosphate.
Manganese toxicity was observed in microculture. The symptoms corresponded to those reported in field studies and increased with Mn concentration but showed little dependence on phosphate levels.
The microculture method confirmed results obtained in greenhouse studies and proved invaluable in studying Mn uptake. This rapid technique provides an effective means for studying young tissue in an environment which is readily controlled and easily manipulated.