Kinetics of phosphate absorption by Stylosanthes spp. in the presence of aluminium

Abstract

The mechanisms of action by which aluminium affects phosphate absorption were evaluated in Stylosanthes guianensis (aluminium‐sensitive) and Stylosanthes macrocephala (aluminium‐tolerant), through kinetic studies with aluminium in pre‐ and co‐treatment. Multiphasic and cooperative models of ions absorption were tested. In the absence of aluminium, S. guianensis, a specie which normaly requires more phosphorus, showed lower rates of phosphate absorption, which was attributed to slower turnover of the carrier. In S. guianensis, the higher rates of absorption may be related to higher turnover and/or higher concentration of the carrier in the roots. Both species showed negative correlation in the absorption of phosphate, but it was more pronounced in S. guianensis. The effect of aluminium seems to be related to the decreased capacity of the carrier to associate with the ion. There may also be a reduction in the turnover of the carrier, which appears to be more pronounced in the tolerant species, reducing phosphate absorption. In the presence of aluminium, the association becomes positive or approaches Michaelis‐Menten kinetics in both species. However, the absorption of phosphate is not increased since there is also a reduction in the capacity of the carrier to bind to the ion.