Erythrosine B influence on sorghum root curvature and wheat leaf rust germling use of extracellular lipids

Abstract

Root curvature response to unilaterally applied calcium (Ca²⁺) in agar was inhibited 81% by erythrosine B (EB) (10 nM) in sorghum [Sorghum bicolor (L.) Moench.] cv Funk G522DR, 70% in cv SC283, and 11% in cv SC574. EB (10 nM) is reported to totally inhibit Ca²⁺‐ATPase, while 10–50 μM EB is required to inhibit H⁺‐ATPase. Therefore, differences in relative concentrations of Ca²⁺‐ATPase and H+‐ATPase exist in the root plasma membranes (PM) of the three cultivars. Carbon dioxide (¹⁴CO₂) production from glycerol‐tri‐(1‐¹⁴C)‐palmitate by wheat (Triticum aestivum L.) leaf rust (Puccinia recondita Rob. ex Desm.) urediospore germlings was inhibited 85% by EB (10 nM).