Abstract
The effects of salt stress(200 mmol L‐1) and calcium (Ca) on the lipid composition and function of plasma membrane and tonoplast vesicles isolated from the roots of barley seedlings (Hordeum vulgare L. Jian 4) have been investigated. Nine days of exposure to 200 mmol L‐1 NaCl resulted in a significant increase in the electrolyte leakage and Na+/K+ ratio in the roots, sheaths, and leaves of barley. The index ofunsaturated fatty acids (IUFA) in plasma membrane (PM) and tonoplast (TP) vesicles, and the levels of galactolipid in TP increased (p<0.01). The contents of phospholipids in PM decreased (p<0.05). In contrast to salt treatment, supplement of Ca2+(17.5 mmol L‐1) obviously increased the phospholipids content, and significantly decreased the levels of galactolipids and IUFA in PM and TP vesicles. The results showed that the alleviating effect of Ca2+ on NaCl induced injury in barley seedlings may be related to the changes of membrane lipid composition including phospholipids, galactolipids, and their fatty acids.
Notes
Supported by National Science Foundation of China (No. 39470074).