38
Views
4
CrossRef citations to date
0
Altmetric
Original Articles

Investigation of ferric iron reduction on the root surfaces of common reeds using an EDTA‐BPDS method

&
Pages 1021-1032 | Published online: 21 Nov 2008
 

Abstract

Graminaceous species usually response to iron (Fe) stress by release of phytosiderophores. But, it remains unclear where Fe3+ is reduced after being chelated with phytosidoerophores. In this investigation, dynamic concentration of Fe2+ and Fe3+ on root surfaces of common reeds [Phragmites australis (Cav.) Trin ex Steudel] was observed using an EDTA‐BPDS (disodium ethylenediamine‐N, N, N’, N'‐tetra acetate and disodiumbathophenanthroline disulfonate) method. The dynamics of Fe2+, Fe3+, and total Fe accumulation on 5 cm tips of roots can be respectively described (in umole g‐1 fresh root weight, FRW) as: [Fe2+]=0.0949t0.21, [Fe3+]=17.9Exp(‐4.96310r0.086) and [Fe]=0.1964t0.28 when the roots were placed in a 0.1 mM ferric citrate solution. Based on the derivatives obtained from the above regression equationss, the accumulation rate (in μmole h‐1 g‐1 FRW) of Fe2+ on the roots was higher than that of Fe3+ during the initial few (<5) minutes, but decreased faster than that of Fe3+. The Fe3+ reduction was also observed in the solution after removing the roots. The reduction rate was 30 times lower than that in the solution with the roots. The results suggested that the reducing power is associated with reductase on the root surfaces.

Notes

The work was carried out at the Department of Soil, Crop and Atmospheric Sciences, Cornell University, Ithaca, NY 14853.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.