An alternative to acetolysis: application of an enzyme-based method for the palynological preparation of fresh pollen, honey samples and bee capsules

Abstract

Acetolysis has been widely used for the preparation of modern pollen samples since its introduction by Erdtman. It has several disadvantages in both safety and resultant changes to the size and, in some cases, structure of pollen grains. The enzymatic technique introduced by Schols and colleagues is tested on a fresh pollen sample, a honey sample and a bee pollen capsule. The technique increases processing time, but is completely non-toxic, easy to complete and comparable in cost to acetolysis. Even very fragile grains are not damaged by this technique.

Keywords:

• enzyme
• cellulase
• pectinase
• melissopalynology
• pollen

Additional information

Notes on contributors

Jennifer M.K. O'Keefe

JEN O'KEEFE is a palynologist and organic petrographer in the department of Earth and Space Sciences at Morehead State University. Her interests lie in fungal palynology, wetland ecosystems from the Cretaceous—Neogene, and improving processing techniques. In addition to her scientific pursuits, Jen is keenly interested in improving K–12 science education and has collaborated with Dr Wymer on many education projects.

Carol L. Wymer

CAROL WYMER is a visiting professor in biology in the Department of Natural Sciences at The University of Findlay. She is presently concentrating on revising instruction in introductory biology courses. Carol has led several educational initiatives in eastern Kentucky and strives to bring authentic science to the masses.