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Ozone: Science & Engineering
The Journal of the International Ozone Association
Volume 44, 2022 - Issue 6
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Research Article

Water-dissolved Ozone Mediates Optimization of Biotechnological Seed Production of Colocasia esculenta (L.) Schott

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Pages 536-544 | Received 12 Aug 2021, Accepted 20 Sep 2021, Published online: 04 Oct 2021
 

ABSTRACT

Taro is a food source for many people in tropical countries. The use of biotechnological techniques for seed production is an alternative that allows obtaining propagation material of higher phytosanitary quality. During the taro in vitro propagation it is of great importance to increase the efficiency of the protocols that are affected mainly by the incidence of microbial contamination and low multiplication coefficients. At the initiation stage, 100% of taro apical meristems sprouted with the immersion in water-dissolved ozone at 2 ppm for 15 min. Furthermore, the microbial contamination was eliminated and the explants showed better quality, increasing the multiplication coefficient in the subsequent subcultures. The disinfection strategy applied allowed to rise the number of taro explants by more than three times, increasing the economic efficiency of the process.

Acknowledgments

The authors thank to Plant Virology Department from The Leibniz Institute (DSMZ) German Collection of Microorganisms and Cell Cultures for the donation of all the reagents used in the ELISA tests.

Disclosure statement

No potential conflict of interest was reported by the author(s).

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