Pilot-Plant-Scale Ozone and PEROXONE Disinfection of Giardia muris Seeded into Surface Water Supplies

Abstract

PEROXONE is an advanced oxidation process for water treatment which is generated by combining ozone and hydrogen peroxide. In this study, surface water supplies were seeded with viable Giardia muris cysts and disinfected by ozone and PEROXONE in the pretreatment columns of a 22.7 L/min pilot plant. Inactivation was examined in two source waters under conditions of varying applied ozone doses (0.5–4.0 mg/L), with and without the addition of hydrogen peroxide; at increasing contact times (3, 6, 9, and 12 min); and with and without added high turbidity (10 and 50 NTU). Approximately 10⁸ viable G. muris cysts were injected into the ozone contactor-column influent. The cysts were collected at the ozone contactor-column effluent and concentrated, and viability was then determined using in vitro excystation.

Key Words:

• Ozone
• Disinfection
• Inactivation
• Giardia lamblia Cysts
• Giardia muris Cysts
• CT Values
• PEROXONE
• Ozone/Hydrogen Peroxide
• Excystation
• Pilot Plant Study
• HPC Bacteria
• T₅₀ Ozone Contact Times