Biomolecule-Assisted Hydrothermal Synthesis ZnSe Nanocrystallites and Its Application as Oligonucleotides Label

Abstract

A simple biomolecule-assisted hydrothermal approach was developed to synthesize ZnSe nanoparticles in this article. As-prepared zinc selenide was characterized by XRD, TEM, FTIR and UV-vis technologies. During the whole procedure, L-glutamic acid acted not only as complexing and capping agent, but a bridging agent between DNA molecule and zinc selenide nanospheres. The ZnSe nanoshperes could be used for the detection of the DNA hybridization. DNA specific-sequence related to PAT gene in the transgenic plants was determined with a detection range from 1.0 × 10⁻¹¹ to 1.0 × 10⁻⁷ mol L⁻¹ and a detection limit of 4.7 × 10⁻¹² mol L⁻¹ (3σ).

Keywords:

• Glutamic acid
• oligonucleotides label
• ZnSe

The authors gratefully acknowledge financial supports from the National Science Foundation of China (20635020), the Doctoral Foundation of the Ministry of Education of China (20060426001) and (20050426003), and the Key Laboratory Foundation of Eco-Chemical Engineering, Ministry of Education of China (KLECE 2007-001).