Fluorescent Vesicular Particles Assembled by Inclusion Complexes Between Cyclodextrins and BPB

Abstract

Vesicular particles based on inclusion complexes between BPB and γ-HB-β-CDs were prepared and characterized for the first time. The morphologies and sizes were confirmed by transmission electron microscopy (TEM), scanning electron microscope (SEM), and dynamic light scattering (DLS). Particularly, these vesicular particles exhibiting clearly fluorescent dots observed by laser confocal scanning microscopy (LCSM) could be alternative candidates as fluorescent probes and labels being applied to cellular staining, labeling, bio-mimicking and drug delivery. The ultraviolet, fluorescence, and nuclear magnetic resonance (NMR) measurements confirmed the existence of stable 1:1 BPB-γ-HB-β-CD complexes in the system. The vesicular particles were assumed to be constructed by orderly self-aggregates of these inclusion complexes.

Keywords:

• Bromophenol blue
• cyclodextrins
• fluorescent
• inclusion complexes
• vesicular particles

Acknowledgments

†Both H. Zhang and Y. Li have made the same contributions to this article.

The authors are grateful to Prof. Ganzuo Li and Prof. Jingcheng Hao from Key Laboratory of Colloid and Interface Chemistry for assistance with the helpful discussions. The authors also thank Dr. Pamela Holt for editing the manuscript for English.

Notes

^a K = intercept/slope.

*Δδ = δ_mixture − δ_BPB.

*Δδ = δ_mixture − δ_γ-HB-β-CD.