Effect of cetyltrimethylammonium bromide and its gemini homologue bis(cetyldimethylammonium)butane dibromide on activity of xanthine oxidase

ABSTRACT

Spectrophotometry is employed to study the effect cetyltrimethylammonium bromide (CTAB) and bis(cetyldimethylammonium)butane dibromide (C₁₆C₄C₁₆Br₂) on the activity of xanthine oxidase (XO), a key enzyme in purine metabolism, at pH 7.4 and 25°C. The spectrophotometric results revealed that the Gemini surfactant interact strongly with the XO than its conventional single chain counterpart and unfold it to a greater extent as compared to CTAB. The effectiveness of the Gemini in interacting/unfolding the XO are justified owing to peculiar structural features of C₁₆C₄C₁₆Br₂ owing to the presence of two charged headgroups and two hydrophobic tails and hence enhanced competence for electrostatic and hydrophobic interactions.

GRAPHICAL ABSTRACT

KEYWORDS:

• Gemini surfactant
• inhibition
• spectrophotometry
• xanthine oxidase

Acknowledgment

We are thankful to the Head Department of Chemistry, University of Kashmir, for providing the laboratory facilities and Principal Govt. Degree College Pulwama for his constant encouragement.