Factors Involved in Formulation of Oily Delivery System for Proteins Based on PEG-8 Caprylic/Capric Glycerides and Polyglyceryl-6 Dioleate in a Mixture of Oleic Acid with Chitosan

Abstract

Systematic experimental work is required to improve knowledge related to the use of oily delivery systems. This work aimed to examine the influence of different molecular weights chitosan on formation and solubilization ability of w/o system of Labrasol, Plurol Oleique, water and oleic acid. Phase diagrams were constructed. Size measurements were performed for each surfactant in oleic acid. Interfacial tension of chitosan was measured between oleic acid and water at pH 1.5 and 6.25. Effect of chitosan on microemulsion size was studied. When used to deliver rh-insulin to diabetic rats, the mixture showed reduction in blood glucose compared to control.

Keywords:

• Chitosan
• insulin
• microemulsion
• oily system
• protein delivery

Acknowledgments

The authors would like to acknowledge the School of Science at Greenwich University for providing the facility for interfacial tension measurements. This work was financially supported by the Jordanian Pharmaceutical Manufacturing Co., Naor, Jordan.

Notes

^a Calculated according to the formula (chitosan MWt in Da/197)/2.

^b Calculated by multiplying the number of ()/chitosan chain by 28.9 × 10¹⁷.