Electrochemical Assay of Methyl Parathion in Homogeneous Reaction Using Water Soluble Films with Immobilized Acetylcholinesterase

Abstract

Water soluble poly (vinyl alcohol) (PVA) was used to prepare the enzyme membranes of acetylcholinesterase (AChE) for electrochemical assaying methyl parathion in water insoluble organic solvent. The immobilized enzyme was separated from electrode for the development of enzyme inhibition-based bioassays. This assay is simple and convenient where enzyme membrane is applicable for single use and the electrode for repeated use. The enzyme membranes can be used handily piece by piece and the released AChE performs catalytic reaction homogeneously. The inhibition percentage of AChE increases with the concentration of methyl parathion ranging from 0.1 to 1 mg/mL.

Keywords:

• Acetylcholinesterase
• electrochemical assay
• methyl parathion
• organophosphate
• square wave voltammetry

Acknowledgments

The authors greatly appreciate the support of the National Natural Science Foundation of China (No. 20975093) and Zhejiang Provincial Natural Science Foundation of China (No. R4100049).