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Abstract
A simple, rapid, sensitive and inexpensive method has been developed for the determination of ethylenediamine (EDA) in European Union food simulants. The method involves precolumn derivatization with ortho-phthaldehyde (OPA) and 2-mercaptoethanol (ME) to obtain a fluorescent derivative. Liquid chromatographic (HPLC) elution was achieved with methanol-ultrapure water (65:35) as mobile phase with a Waters Spherisorb 5 mu;m ODS 2 column. Fluorescence detection (FD) was performed at 330 nm (excitation wavelength) and 450 nm (emission). Total chromatographic analysis time was < 10 min. The proposed method was validated by checking linearity, detection and quantification limits, and precision. Relative recovery rates were of about 100% because samples and standard-spiked blanks were processed in the same way. Method precision (RSD < 6%) was satisfactory and the quantification limit (0.25 mg l-1) indicated that specific migration limit for EDA in EU food simulants (12 mg kg-1) can be easily controlled. When the validated method was applied to epoxy-amine formulations used for can coatings under different curing conditions, EDA migration was < 1.4 mg l-1.
