Residues of doxycycline and oxytetracycline in eggs after medication via drinking water to laying hens

Abstract

Doxycycline (DOTC) and oxytetracycline (OTC) were dissolved in drinking water (0.5 g/l) and supplied to laying hens for 7 consecutive days. Eggs laid were collected daily during and after medication, and the antibiotic concentrations in the yolk and albumin were determined by the cup‐plate method with Bacillus cereus var. mycoides ATCC 11778. The concentrations of both antibiotics were increased in yolk day by day with the advance in medication, reached peaks 2 days after withdrawal and then declined gradually. Mean peak concentrations in the yolk were 6.70 μg/g for DOTC and 1.42 μg/g for OTC. Peak concentrations in the albumin occurred in the middle stage of medication, where the mean values were 12.24 μg/g for DOTC and 1.03 μg/g for OTC. DOTC was detected in albumin until 24 days after withdrawal and for 2 days more in yolk than in albumin. OTC was detected in yolk until 9 days after withdrawal. The depletion period of OTC was shorter for the albumin, where the residue disappeared in all eggs 6 days after withdrawal. In spite of similarities between DOTC and OTC in structure, DOTC was deposited in higher concentrations and lasted for a longer period in eggs. This characteristic was considered due to its greater lipophilicity, closely correlated with oral absorption and tissue penetration.

Keywords:

• doxycycline
• oxytetracycline
• residues
• eggs

Notes

Present addresses: National Veterinary Assay Laboratory, 1–15–1 Tokura, Kokubunji‐shi, Tokyo 185, Japan—to whom correspondence should be addressed;

Japan Food Research Laboratories, 52–1 Motoyoyogi‐cho, Shibuya‐ku, Tokyo 151, Japan;

Japan International Cooperation Agency, Shinjuku Mitsui Bldg., 2–1–2 Nishi‐shinjuku, Shinjuku‐ku, Tokyo 163, Japan