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Abstract
A method for the rapid and quantitative determination of aflatoxin B1 from small quantities of liver, around 1–2 g, is described. The extraction procedure involves acidification to pH 2 of the aqueous liver homogenates, extraction with chloroform: acetone and HPLC‐fluorimetric detection after derivatization with trifluoroacetic acid. Quantitative recovery of aflatoxin B1 from chick liver was achieved and detection at levels of 0.2–1 ppb was proved feasible. The aflatoxin B1 concentration in chick liver after oral administration is also shown.