Effect of alginate composition and gelling cation on micro-bead swelling

Abstract

Purpose. The purpose of this study was to determine the roles of alginate composition and gelling cations on bead swelling, which affects its durability. Method. Using a 2-channel droplet generator, microspheres were generated with 1.5% solutions of low viscosity high-mannuronic acid (LVM), medium viscosity high-mannuronic acid (MVM), low viscosity high-guluronic acid (LVG) and medium viscosity high-guluronic acid (MVG) alginate. They were gelled by cross-linking with 1.1% solution of either BaCl₂ or CaCl₂. The diameters of the micro-beads were measured and recorded on day 0. The micro-beads were subsequently washed and incubated in saline at 37°C for 2 weeks with size assessment every 2 days. The data were normalized by calculation of the percentage change from control (day 0) for all groups of micro-beads. Results. Diameters of all beads were between 550 and 700 µm on day 0. Viscosity had no effect on swelling of Ba⁺⁺- and Ca⁺⁺-alginate micro-beads. Ca⁺⁺-alginate micro-beads were more prone to swelling than the corresponding Ba⁺⁺-alginate beads. High G-Ba⁺⁺ beads had only a modest increase in size over time, in contrast to the high M-Ba⁺⁺. Conclusion. Alginate composition and the gelling cation have significant effects on bead swelling.

Key Words: :

• Alginate
• microcapsules
• cations
• swelling
• islets
• transplantation