Formulation, characterization and evaluation of rotavirus encapsulated PLA and PLGA particles for oral vaccination

Abstract

Polylactide (PLA) and polylactide-co-glycolide (PLGA) particles entrapping rotavirus (strain SA11) were formulated using a solvent evaporation technique. To minimize denaturation of viral antigen during the emulsification process, serum albumin was used as a stabilizer. Use of NaHCO₃ and sucrose during the primary emulsification step resulted in uniform stabilized particles entrapping rotavirus. Sonication during the primary emulsion and homogenization during the secondary emulsion process resulted in particles of sizes 2–8 μm, whereas nanoparticles were formed when sonication was used during both primary and secondary emulsion processes. Scanning electron and atomic force microscopy showed uniform pores and roughness throughout the polymer particle surface. Single dose oral immunization with 20 μg of antigen entrapped in PLA particles elicited improved and long-lasting IgA and IgG antibody titer in comparison to the soluble antigen. The study shows results illustrating the usefulness of polymeric microparticles as a potential oral delivery system for rotavirus vaccine.

• Microparticle
• biodegradable polymers
• rotavirus
• immune response
• IgA and IgG antibody titer

Acknowledgement

One of the authors (Bismita Nayak) acknowledges the Council for Scientific and Industrial Research, New Delhi, India for providing a Research Fellowship.