Surface modified kokum butter lipid nanoparticles for the brain targeted delivery of nevirapine

Abstract

Aim: The present work investigates the efficacy of Polysorbate 80(P80) coated Kokum butter (KB) solid lipid nanoparticles (P80NvKLNs) for the brain targeted delivery of Nevirapine (Nv).

Methods: Solid lipid nanoparticles (SLNs) were prepared by nanoprecipitation technique and evaluated for drug excipient compatibility studies, z- average particle size (nm), zeta potential (mv), percentage drug entrapment efficiency (%EE), surface morphology and in-vitro drug release properties. The in-vivo biodistribution and brain targeting efficiency of nanoparticles were studied in healthy male Wistar rat (150–200 g).

Results: P80NvKLNs were found to be smooth surfaced, spherical shaped having average particle size of 177.80 ± 0.82 nm, zeta potential of −8.91 ± 4.36 mv and %EE of 31.32 ± 0.42%. P80NvKLNs remained in blood circulation for 48 h maintaining a sustained release in brain for 24 h (p < 0.05).

Conclusion: The study proves the efficacy of Polysorbate 80 coated Kokum butter nanoparticles for brain-targeted delivery of drugs providing ample opportunities for further study.

KEYWORDS:

• Blood-brain barrier (BBB)
• SLNs
• kokum butter
• nevirapine
• nanoprecipitation
• in-vivo biodistribution

Acknowledgement

The authors are thankful to Dibrugarh University, Girijananda Choudhury Institute of Pharmaceutical Sciences (GIPS), Indian Institute of Advances in Science and Technology (IASST), Northeast Hill University (NEHU), College of Veterinary Sciences, AAU and Guwahati Biotech Park for their support in carrying out the work.

Disclosure statement

The author has no conflict of interest.