215
Views
4
CrossRef citations to date
0
Altmetric
Original Articles

Preparation, characterisation and in vitro antibacterial property of ciprofloxacin-loaded nanostructured lipid carrier for treatment of Bacillus subtilis infection

ORCID Icon, , , , , , , ORCID Icon & show all
Pages 32-42 | Received 18 Sep 2018, Accepted 11 Feb 2019, Published online: 04 Mar 2019
 

Abstract

Context: In this study, controlled ciprofloxacin (CIPRO) nanostrustructured lipid carriers of Precirol® ATO 5/Transcutol® HP (batch A) and tallow fat/Transcutol® HP (batch B) was carreid out. Objective: The aim was to improve solubility and bioavailability of CIPRO.

Objective: Study of controlled ciprofloxacin (CIPRO) nanostructured lipid carriers of Precirol® ATO 5/Transcutol® HP (batch A) and tallow fat/Transcutol® HP (batch B).

Methods: CIPRO concentrations C1–5 (0.0, 0.2, 0.5, 0.8, and 1.0% w/w) as AC1–5 and BC1–5 were prepared by hot homogenisation and characterised by zetasizer, differential scanning calorimetry, Fourier transform infra-red spectroscopy, in vitro drug release and growth inhibitory zone diameter (IZD) on agar-seeded Bacillus subtilis.

Results: AC5 achieved polydispersed particles of ∼605 nm, 92% encapsulation efficiency (EE) and –28 mV similar to BC5 (∼789 nm, 91% EE, and –31 mV). Crystallinity indices (AC5 and BC5) were low at 3 and 5%, respectively. CIPRO release in AC5 was ∼98% in SGF (pH 1.2) and BC5 similarly ∼98% in SIF (pH 6.8).

Conclusions: AC5 had superior growth inhibition of B. subtilis at lower concentration (1.2 µg/mL) than BC5 and CIPRO controls; hence could serve as possible sustained delivery system of CIPRO.

Acknowledgements

We appreciate the assistance of Mr. Kalu Ogboso for antimicrobial studies and Dr. Chiara Rossi for SEM analysis. We thank Phospholipid GmbH Köln, Germany, BASF Ludwigshafen, Germany and Gattefossé, St. Priest, France for gift samples of Phospholipon® 90G; Poloxamer® 188 and Solutol® HS 15; and Precirol® ATO 5 and Transcutol® HP, respectively.

Disclosure statement

No potential conflict of interest was reported by the authors.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.