In vitro survival of Bifidobacterium bifidum microencapsulated in zein-coated alginate hydrogel microbeads

Tahreem RiazState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; ;International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China; ;Department of Food, Nutrition and Home Sciences, National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan; Correspondence[email protected]

http://orcid.org/0000-0003-1855-4602

Muhammad Waheed IqbalState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; ;International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China; ;Department of Food, Nutrition and Home Sciences, National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan;

Muhammad SaeedDepartment of Food, Nutrition and Home Sciences, National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan;

Iqra YasminDepartment of Food, Nutrition and Home Sciences, National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan; ;Department of Food Science and Technology, University of Nebraska, Lincoln, NE, USA; ;Department of Diet and Nutritional Science, Faculty of Health and Allied Science, Imperial College of Business Studies, Lahore, Pakistan; ;Department of Food Science and Technology, Government College Women University, Faisalabad, Pakistan

Hinawi A. M. HassaninState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; ;International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China;

Shahid MahmoodState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; ;International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China; ;Department of Food, Nutrition and Home Sciences, National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan;

Abdur RehmanState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; ;International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China; ;Department of Food, Nutrition and Home Sciences, National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan;

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Abstract

The Bifidobacterium bifidum susceptibility in gastrointestinal conditions and storage stability limit its use as potential probiotics. The current study was design to encapsulate B. bifidum using sodium alginate (SA, 1.4% w/v) and different concentration of zein as coating material, that is, Z₁ (1% w/v), Z₂ (3% w/v), Z₃ (5% w/v), Z₄ (7% w/v), Z₅ (9% w/v). The resultant microbeads were further investigated for encapsulation efficiency, survival in gastrointestinal conditions, release profile in intestinal fluid, storage stability and morphological characteristics. The highest encapsulation efficiency (94.56%) and viable count (>10⁷ log CFU/g) was observed in Z₄ (7% w/v). Viable cell count of B. bifidum was >10⁶ log CFU/g in all the zein-coated microbeads as compare to free cells (10³ log CFU/g) and SA (10⁵ log CFU/g) at 4 °C after 32 days of storage. Therefore, B. bifidum encapsulated in zein-coated alginate microbeads present improved survival during gastric transit and storage.

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Acknowledgements

The authors are very thankful to University of Agriculture, Faisalabad, Ayub Agriculture Research Institute, Pakistan and Jiangnan University, Wuxi, China for their support during this research.

Disclosure statement

No potential conflict of interest was reported by the authors.

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In vitro survival of Bifidobacterium bifidum microencapsulated in zein-coated alginate hydrogel microbeads

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In vitro survival of Bifidobacterium bifidum microencapsulated in zein-coated alginate hydrogel microbeads

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