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Original Articles

Comparison of Virosome vs. Liposome as drug delivery vehicle using HepG2 and CaCo2 cell lines

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Pages 263-275 | Received 06 Oct 2020, Accepted 08 Mar 2021, Published online: 25 Mar 2021
 

Abstract

Aim

The present work involves encapsulation of herbal drug nanocurcumin into the virosomes and compared with a liposome in terms of their in vitro anti-proliferative, anti-inflammatory, and anti-migratory efficacy.

Methods

The anti-proliferative, anti-inflammatory, and anti-migratory efficacy of virosome and liposome were compared in HepG2 and CaCo2 cells by using MTT, Nitric oxide scavenging, and Wound healing assay, respectively.

Results

Size of the optimised NC-Virosome and NC-Liposome was 70.06 ± 1.63 and 265.80 ± 1.64 nm, respectively. The prepared NC-Virosome can be stored at −4 °C up to six months. The drug encapsulation efficiency of NC-Virosome and NC-Liposome was found to be 84.66 ± 1.67 and 62.15 ± 1.75% (w/w). The evaluated minimum inhibitory concentration (IC50 value) for NC-Virosome was 102.7 μg/ml and 108.1 μg/ml, while NC-Liposome showed 129.2 μg/ml and 160.1 μg/ml for HepG2 and CaCo2 cells, respectively. Morphological examination depicts detachment of the cells from substratum after exposure to NC-Virosome for 48 h.

Conclusion

The prepared NC-Virosome provides remarkable in vitro efficacy in both the cell lines with site-specific drug-targeting potential as compared to the liposome, results proved its potential as a drug delivery vehicle for future therapy with reduced toxicity.

Acknowledgements

The authors acknowledge the funding given to Varun Kumar, from the Department of Biotechnology (DBT/JRF/BET/I/2017/AL/319), New Delhi, and the Government of India. The authors also like to show our gratitude to Dr. Ashok Chauhan, Founder of President Amity University for their continuous encouragement and support.

Human and animal rights

No Humans and animals were used for studies.

Disclosure statement

There is no conflict of interest in the present study.

Additional information

Funding

This work was supported by Department of Biotechnology, Ministry of Science and Technology.

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