Abstract
Liposomes (LS) were prepared using chitosan-epigallocatechin gallate (CE) conjugate (0.1 and 0.5%, w/v) and soy phosphatidylcholine (SPC)/cholesterol as a lipid phase (LP) (30 and 60 µmol mL−1). The encapsulation efficiency (EE), particle diameter, zeta potential, and polydispersity index of LS were observed. The highest EE (76.96%) was found when LS was prepared using 0.5% (w/v) of CE conjugate and 60 µmol mL−1 of LP (CELP-60-0.5) (p < 0.05). FTIR analysis showed the interaction between choline present in SPC and OH-groups of CE conjugate. The phase transition temperature of CELP-60-0.5 was 134.67 °C, higher than other samples (p < 0.05). CELP-60-0.5 showed inhibitory action against Gram-positive and Gram-negative bacteria. Higher retention of antioxidant and antimicrobial activities of CELP-60-0.5 was observed than unencapsulated CE conjugate sample when stored for 28 days at 30 °C (p < 0.05). LS might be used as an efficient vesicle for maintaining bioactivities of CE conjugate, plausibly when used as a preservative in foods.
Author contributions
Ajay Mittal: data curation; investigation; methodology; writing‐original draft. Avtar Singh: conceptualisation; data curation; writing‐review and editing. Soottawat Benjakul: conceptualisation; funding acquisition; resources; supervision; writing‐review and editing.
Acknowledgements
This research was supported by National Science, Research and Innovation Fund (NSRF) and Prince of Songkla University (Grant No. AGR6505155M). The International Research Network program from the National Research Council of Thailand (NRCT) was acknowledged. Chair professor grant (P-20-52297) was also acknowledged.
Disclosure statement
The authors declare that they have no conflict of interest.