Construction and evaluation of an antioxidant synergistic system containing vitamin C and vitamin E

Abstract

Aims

The aim of this study is to develop a liposome that could exert unparalleled antioxidant effects. In the present study, a vitamin C (VC)/vitamin E (VE)-co-loaded multivesicular liposome (VCVE-MVL) was constructed.

Methods

Vitamins were encapsulated in soybean phosphatidylcholine (SPC) and cholesterol (CHO) by multi-emulsification method. The concentration of VC was determined by Fast Blue method. The concentration of VE was determined by high performance liquid chromatography (HPLC). Vitamin antioxidant capacity in vitro and in vivo was determined using β-carotene bleaching.

Results

VCVE-MVL with particle diameter of 848.55 ± 0.29 nm and SPAN of 0.16 ± 0.11 were obtained. The encapsulation efficiency of VC reached 48.51% (w/w)±0.15. Compared with VC/VE solution, VCVE-MVL had a higher permeation efficiency. In addition, the in vitro and ex-vivo antioxidant tests verified the adequate antioxidant activity of VCVE-MVL.

Conclusions

In conclusion, the antioxidant synergistic system we constructed and demonstrated its potential applications in the cosmetics industry.

Keywords:

• Vitamin C
• vitamin E
• liposomes
• transdermal permeability
• antioxidant

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The present study was supported by the Chongqing Basic and Frontier Research Project (grant no. CSTC2018jcyjAX0126), the Science and Technology Research Program of Chongqing Municipal Education Commission (grant no. KJQN202000407), the Chongqing College Student Innovation and Entrepreneurship Project (grant no. CXSY201856), and the Program of Postgraduate Joint Training Base of Chongqing (Chongqing Medical University & Yaopharma Co., Ltd joint training base for postgraduate in pharmacy).