Abstract
Aim
To modify the techno-functional properties of mung bean protein isolate (MBPI) by high-intensity pulsed electric field (HIPEF) treatment and to apply the treated MBPI for encapsulation of Asian seabass oil (ASO).
Methods
MBPI was prepared using isoelectric precipitation. HIPEF was applied to MBPI solutions at 25 kV/cm with varying pulse numbers (0–400). Physicochemical properties and structure of MBPI were assessed. ASO microcapsules prepared using HIPEF-treated protein as wall material was characterised and tested for storage stability.
Results
Solubility, surface hydrophobicity, total sulfhydryl content, and emulsifying property of MBPI increased and β-sheets and α-helix were altered after HIPEF treatment at pulse number of 300. ASO microcapsules possessing spherical shape with surface indentations had EE of 72.07 ± 5.08%. ASO capsules had lower lipid oxidation than the control during storage.
Conclusion
HIPEF improved techno-functional properties of treated MBPI. Treated MBPI could be used as wall material for encapsulation of fish oils.
Acknowledgements
This research was supported by National Science, Research and Innovation Fund (NSRF) and Prince of Songkla University. Postdoctoral Fellowship from Prince of Songkla University, Thailand to Dr. Saqib Gulzar. Prachayajarn Scholarship is also acknowledged. The authors are also grateful to NSTDA for the Chair Professor Grant.
Author contributions
Saqib Gulzar: data curation; formal analysis; investigation; methodology; writing – original draft. Mohamed Tagrida: resources; data curation. Umesh Patil: resources; data curation. Lukai Ma: validation; data curation. Bin Zhang: validation; data curation. Soottawat Benjakul: conceptualisation; supervision; validation; writing – review and editing.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
Data available within the article or its supplementary materials.